Instructions - for users of Microsoft WINDOWS

If you want to submit your mtFullSequence to GenBank you can use this Javascript program to make your own submission file.

BUT NOTE: YOU BE A CUSTOMER OF YSEQ AND HAVE A SEQUENCE OF HIGH STANDARD.

And, using this program you can only make a submission for yourself.

(If you want to make a submission of the mtFullSequence results for another person - I suggest you email me and discuss how to proceed.)

 

A little computer skill is needed - but if you take it carefully and slowly the process of making a submission file is not difficult.

So to proceed ....

 

1. Preparing the FASTA file.

   c. Using Windows Explorer display the directory contaning your FASTA file.

   d. Rename the file xxx.TXT, by rightclicking on the filename and using RENAME.

       (To the question, Are you sure you want to change it? Answer YES)

   e. Now leftclick on the xxx.TXT filename and the file should open in NOTEPAD or WORDPAD.

   f. Minimise this NOTEPAD window as you will need it later.

 

2. Entering the 5 other inputs needed for the program.

   a. DATE: In the above form change the date to "today's date".

   b. FILENAME: In the next box enter your surname - whilst any name can be used, I do suggest that you use your surname.

       (Note: Your surname will NOT appear on the final GenBank page.)

   c. ETHNICITY: Enter your choice here. May I suggest this is kept simple AND do make sure of the spelling.

       Suitable examples could be: English/Scottish/Jewish/Askenazi Jew/Native American.

       European/American/African/Caucasian/Asiatic are not very helpful and often it is appropriate to leave this entry blank.

   d. LOCATION: Enter your choice here. Again, keep it simple AND take care with the spelling.

       Try to be helpful and use the format 'Country: City'. If in any doubt leave it blank.

   e. HAPLOGROUP: Enter the haplogroup here. Check the label from www.phylotree.org, if needed.

 

3. Transferring the FASTA file.

   a. Display your xxx.TXT file and leftclick in the window to show the cursor.

   b. Use CTRL-A to highlight the contents of the file, followed by CTRL-C to capture the contents.

   c. Again minimise the NOTEPAD window and display the entry form once again.

   d. Move the cursor to the FASTA file entry box and leftclick to enter the cursor and use CTRL-V to paste your FASTA file into the box.

       At this stage the FASTA file box should contain a copy of the FASTA file

       with the first line reading similar to '>' and the bottom lines ending with 'CATCACGATG'.

       DO TAKE CARE SO AS NOT TO CORRUPT THE CONTENTS OF THIS BOX !

 

4. Executing the Javascript program.

   a. It is now time to execute the program, so press SUBMIT.

       And ... the text of the submission file should appear in the lower box.

       By all means look at the submission file, and again DO TAKE CARE SO AS NOT TO CORRUPT THE CONTENTS OF THIS BOX !

       And if you have any concerns, press CLEAR BOXES and repeat the procedure.

 

5. Transferring the Submission file.

   a. Note the Submission file starts with    Seq-submit ::= {    and finishes with    'H } } } }

   b. Next move the cursor to the Submission file box and leftclick to enter the cursor.

   c. And, use CTRL-A, and CTRL-C to capture the all the text of the submission file

   d. Now open a new NOTEPAD window by using START, ALL PROGRAMS, ACCESSORIES, NOTEPAD

   e. Move the cursor to the new NOTEPAD window, leftclick to enter the cursor and use CTRL-V to paste your submission file into the box.

   f. Check once more that the Submission file starts with    Seq-submit ::= {    and finishes with    'H } } } }

   g. Now use FILE and SAVE AS ... to save the submission file using the same filename you used at 2b, BUT MOST IMPORTANTLY use the filetype .sqn

      For example: If the filename was

      logan

      save the file ALL IN LOWER CASE characters as

      logan.sqn

      Again make sure of which directory you are using.

   h. Now open the directory and check you can find the file.

      And, if the filename appears as   xxx.sqn.txt   use RENAME to change it to   xxx.sqn

 

6. Emailing GenBank.

   a. Create a new email to: gb-sub@ncbi.nlm.nih.gov

   b. Enter a subject, such as 'GenBank Submission - xxx.sqn'

   c. And at this stage attach your    xxx.sqn    file by using 'Insert' and 'File as attachment...' (or whatever is appropriate on you email program).

   d. In the body of the email write something like:

      Susan Schafer

      I attach my submission file for GenBank, named 'xxx.sqn'.

      It is for my mitochondrial DNA sequence and the sequence has not been published in any other database.

      My YSEQ ID is 'xxxxx', and I accept it will appear on my GenBank page.

      Thank you

      (and your full name)

   e. Please note that normally your surname will not appear on the final GenBank page.

   f. Finally: when you are totally happy, send the email with its attachment.

 

7. The process afterwards.

   a. After submitting your email you will get several emails from GenBank.

   b. A receipt will be sent almost immediately.

   c. Followed a couple of days later by an email asking you to re-confirm the sequence is yours.

      Reply to this email to say you are happy to proceed.

   d. An email giving your Accession number.

   e. Finally some days later, an email containing a 'flat file' - which is a 'proof' copy of your page - and your publication data.

      Make a final check of the 'flat file' to see it contains the correct information.

 

8. Publication.

   a. On publication date your sequence should be viewable on GenBank, by entering your Accession number.

   b. However, it will be 2-4 days before the sequence is indexed and can be found

      by using a search string, such as:    "Homo sapiens" [ORGANISM] "complete genome" mitochondrion

 

9. Revising a GenBank page

      It is to be hoped that a GenBank page never needs revision, but there are several reasons why it might.

      However, revision should NOT be undertaken just to change the haplogrouping as new subgroups are defined.

      But if there is an overt error on the GenBank page, or YSEQ amends the results, then revision will be appropriate.

   So if revision is necessary the required steps will be:-

   a. Follow the steps above, correcting the error, and make a fresh submission file, with the filename, for example:

      logan2

   b. Create a new email to: gb-admin@ncbi.nlm.nih.gov

   b. Enter a subject, such as 'Revision of my GenBank Submission - Accession Number XXnnnnnn'

   c. And at this stage attach your    xxx2.sqn    file by using 'Insert' and 'File as attachment...' (or whatever is appropriate on you email program).

   d. In the body of the email write something like:

      Susan Schafer

      I am sorry for the inconvenience but my earlier submission for my GenBank entry with Accession Number XXnnnnnn needs to be revised.

      I attach a corrected submission file, named 'xxx2.sqn'.

      This is required as the earlier submission had an error (specify, eg. YSEQ has amended the sequencing).

      The submission file is for my mitochondrial DNA sequence and the sequence has not been published in any other database.

      Thank you

      (and your full name)

   e. And send the email.

 

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Revision history for this program

12 Mar 2017   Version 1.000   First YSEQ version

 

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IF YOU HAVE ANY QUESTIONS   -   CONTACT ME ON:   ianlogan22@btinternet.com